Optimizing PCR reactions for gene amplification Part II

Here is a slightly modified one for those troublesome genes that won’t amplify or if you get lots of smearing.

Basic Master Mix recipe for AmpliTAQ gold

for 15 µL reaction

(volumes in µL)
H2O 9.57
10x PCR Buffer 1.5
100x BSA 0.125
Forward Primer 0.1
Reverse Primer 0.1
TAQ 0.15
MgCl2 1.5
DMSO 1.5

Advertisements
This entry was posted in Uncategorized and tagged , , , . Bookmark the permalink.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s