So I am wrangling with getting these literature based PKS I primers working for my PhD project. Finally success!!! PKS I Primers degKS2F 5’-GCIATGGAYCCICARCARMGIVT degKS2R 5’- GTICCIGTICCRTGISCYTCIAC 760 bp fragment Schirmer, A., R. Gadkari, C. D. Reeves, F. Ibrahim, E. F. DeLong, … Continue reading
Just got back from an amazing time in the Parashants! Will post an update shortly.
For those that are interested in opensource science I am over on figshare as well. Just posted GC content and genome size plot from some data I mined from NCBI awhile back. http://figshare.com/articles/GC_Content_vs_Genome_Size_of_4231_Bacteria_Genomes/97275