Author Archives: Tattooed Science
I get torn between putting out work here, on github and on google+ . I think each site serves a slightly different purpose. Github for code, google+ for a narrower audience, and here maybe for a scratch space for writing … Continue reading
So I am wrangling with getting these literature based PKS I primers working for my PhD project. Finally success!!! PKS I Primers degKS2F 5’-GCIATGGAYCCICARCARMGIVT degKS2R 5’- GTICCIGTICCRTGISCYTCIAC 760 bp fragment Schirmer, A., R. Gadkari, C. D. Reeves, F. Ibrahim, E. F. DeLong, … Continue reading
Just got back from an amazing time in the Parashants! Will post an update shortly.
For those that are interested in opensource science I am over on figshare as well. Just posted GC content and genome size plot from some data I mined from NCBI awhile back. http://figshare.com/articles/GC_Content_vs_Genome_Size_of_4231_Bacteria_Genomes/97275
I like this sketch I did. This shows the spatial relationship between several cave pools in cross section to the geologic structures in the area. The numbers with the arrows are the strikes of the flowstone and rimstone dams. The … Continue reading
Here is a slightly modified one for those troublesome genes that won’t amplify or if you get lots of smearing. Basic Master Mix recipe for AmpliTAQ gold for 15 µL reaction (volumes in µL)H2O 9.5710x PCR Buffer 1.5100x BSA 0.125Forward … Continue reading