Tag Archives: PCR

Primers working!!!

So I am wrangling with getting these literature based PKS I primers working for my PhD project. Finally success!!! PKS I Primers degKS2F 5’-GCIATGGAYCCICARCARMGIVT  degKS2R 5’- GTICCIGTICCRTGISCYTCIAC 760 bp fragment Schirmer, A., R. Gadkari, C. D. Reeves, F. Ibrahim, E. F. DeLong, … Continue reading

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Optimizing PCR reactions for gene amplification Part II

Here is a slightly modified one for those troublesome genes that won’t amplify or if you get lots of smearing. Basic Master Mix recipe for AmpliTAQ gold for 15 µL reaction (volumes in µL)H2O 9.5710x PCR Buffer 1.5100x BSA 0.125Forward … Continue reading

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Optimizing PCR reactions for gene amplification – Master Mix

OVERVIEW If you are working in the geomicrobiology field or a geologist (or just curious) )who is interested in geomicro you will run into PCRs (Polymerase Chain Reaction) at some point in time. Basically the PCR lets you target a … Continue reading

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